Isolation of centrosomes from cultured cells.

In Methods Mol, Biol. 2011;777:107-16.   


Chromatin Beads.

Spindle Assembly in Xenopus Egg Extracts.

This protocol is essentially as described by Sawin and Mitchison “Mitotic spindle
assembly by two different pathways in vitro“, J Cell Biol. 1991 Mar;112(5):925-40.

Labelling Porcine Tubulin.

Preparing CSF-extract from Xenopus Eggs.

This protocol is essentially as described by Murray in „Cell cycle extracts“, Methods
Cell Biol. 1991;36:581-605.

XMAP215 Expression & Purification.